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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
29/09/2014 |
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Actualizado : |
24/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33. |
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ISSN : |
0717-3458 |
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DOI : |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped... Presentar Todo |
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Thesagro : |
CITRUS; CYDIA. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02405naa a2200241 a 4500
001 1050705
005 2021-06-24
008 2012 bl uuuu u00u1 u #d
022 $a0717-3458
024 7 $ahttp://dx.doi.org/10.2225/vol15-issue3-fulltext-4$2DOI
100 1 $aFEDERICI, M.
245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource]
260 $c2012
520 $aABSTRACT. Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCITRUS
650 $aCYDIA
700 1 $aMARCONDES, J.A.
700 1 $aPICCHI, S.C.
700 1 $aSTUCHI, E.S.
700 1 $aFADEL, A.L.
700 1 $aLAIA, M.L.
700 1 $aLEMOS, M.V.F.
773 $tElectronic Journal of Biotechnology, 2012$gv.15, no.3, p.1-33.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
09/11/2017 |
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Actualizado : |
12/12/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CÉSPEDES-LEÓN, C.; BALZARINI, M.; ZOPPOLO, R.; ZARZA, H.; RODRÍGUEZ, E.M.; GRANVAL, N.I.; AMADO, I. |
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Afiliación : |
CECILIA CÉSPEDES-LEÓN, INIA CL (Instituto de Investigaciones Agropecuarias de Chile); MÓNICA GRACIELA BALZARINI, Universidad Nacional de Córdoba, CONICET, Argentina; ROBERTO JOSE ZOPPOLO GOLDSCHMIDT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; HUGO ALBERTO ZARZA, Instituto Paraguayo de Tecnología Agropecuaria, Paraguay; ELSA MIRTA MARGARITA RODRÍGUEZ, Universidad Nacional de Mar del Plata, Argentina; NÉLIDA ISABEL GRANVAL, Instituto Nacional de Tecnología Agropecuaria, Argentina; IVÁN AMADO TORRICO, Instituto Nacional de Innovación Agropecuaria y Forestal, Plurinational State of Bolivia. |
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Título : |
Successful organic production practices in the Southern Cone. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Sustainable Agriculture Research, 2017, v.6 (3): 26-34. |
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ISSN : |
1927-050X (Print) // 1927-0518 (Online) |
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DOI : |
10.5539/sar.v6n3p26 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: April 3, 2017; Accepted: May 13, 2017; Online Published: June 1, 2017. |
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Contenido : |
ABSTRACT.
The growth of demand of organic products has increased the interest of growers all around the World. To reach a sustainable organic production system, growers have to go through a long and difficult process. Eighty-one successful organic production systems in the Southern Cone of America were evaluated regarding management practices, such as disease control, soil fertility, and biodiversity management. The aim of the study was to determine associations between production techniques, farmer training, experience in organic agriculture, yields obtained, and the contribution of organic production to the profitability of farmers. A multivariate analysis was performed to characterize the variability between systems, correlate the management variables and explain economic sustainability. The economic sustainability of farmers was highly correlated with self-production of fungicides and insecticides, use of permitted commercial fertilizers, organic matter application, the use of natural enemies, commercially permitted fungicides and insecticides and the implementation of preventive practices for disease management. Pest monitoring correlated significantly with farmer training and sustainable practices implementation. Farmer?s perception on the importance of crop diversity in organic production correlated with the importance of external inputs independence, green manure, cover crop, and the experience of farmers in organic production. Farmers who implemented more management practices had yield losses below 20% of total production and a gross margin of organic farming of more than 1.5 times the minimum wage of the country than farmers that implemented few or no management practices.
Copyright © Canadian Center of Science and Education MenosABSTRACT.
The growth of demand of organic products has increased the interest of growers all around the World. To reach a sustainable organic production system, growers have to go through a long and difficult process. Eighty-one successful organic production systems in the Southern Cone of America were evaluated regarding management practices, such as disease control, soil fertility, and biodiversity management. The aim of the study was to determine associations between production techniques, farmer training, experience in organic agriculture, yields obtained, and the contribution of organic production to the profitability of farmers. A multivariate analysis was performed to characterize the variability between systems, correlate the management variables and explain economic sustainability. The economic sustainability of farmers was highly correlated with self-production of fungicides and insecticides, use of permitted commercial fertilizers, organic matter application, the use of natural enemies, commercially permitted fungicides and insecticides and the implementation of preventive practices for disease management. Pest monitoring correlated significantly with farmer training and sustainable practices implementation. Farmer?s perception on the importance of crop diversity in organic production correlated with the importance of external inputs independence, green manure, cover crop, and the experience of farmers in organic production. Farmers who implemented more management... Presentar Todo |
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Palabras claves : |
BIODIVERSITY; PREVENTIVE PRACTICES; SOIL ORGANIC MATTER; SUSTAINABLE MANAGEMENT; WEED CONTROL. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7926/1/67319-249314-1-PB.pdf
http://www.ccsenet.org/journal/index.php/sar/article/view/67319/37283
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Marc : |
LEADER 02688naa a2200289 a 4500
001 1057748
005 2018-12-12
008 2017 bl uuuu u00u1 u #d
022 $a1927-050X (Print) // 1927-0518 (Online)
024 7 $a10.5539/sar.v6n3p26$2DOI
100 1 $aCÉSPEDES-LEÓN, C.
245 $aSuccessful organic production practices in the Southern Cone.$h[electronic resource]
260 $c2017
500 $aArticle history: Received: April 3, 2017; Accepted: May 13, 2017; Online Published: June 1, 2017.
520 $aABSTRACT. The growth of demand of organic products has increased the interest of growers all around the World. To reach a sustainable organic production system, growers have to go through a long and difficult process. Eighty-one successful organic production systems in the Southern Cone of America were evaluated regarding management practices, such as disease control, soil fertility, and biodiversity management. The aim of the study was to determine associations between production techniques, farmer training, experience in organic agriculture, yields obtained, and the contribution of organic production to the profitability of farmers. A multivariate analysis was performed to characterize the variability between systems, correlate the management variables and explain economic sustainability. The economic sustainability of farmers was highly correlated with self-production of fungicides and insecticides, use of permitted commercial fertilizers, organic matter application, the use of natural enemies, commercially permitted fungicides and insecticides and the implementation of preventive practices for disease management. Pest monitoring correlated significantly with farmer training and sustainable practices implementation. Farmer?s perception on the importance of crop diversity in organic production correlated with the importance of external inputs independence, green manure, cover crop, and the experience of farmers in organic production. Farmers who implemented more management practices had yield losses below 20% of total production and a gross margin of organic farming of more than 1.5 times the minimum wage of the country than farmers that implemented few or no management practices. Copyright © Canadian Center of Science and Education
653 $aBIODIVERSITY
653 $aPREVENTIVE PRACTICES
653 $aSOIL ORGANIC MATTER
653 $aSUSTAINABLE MANAGEMENT
653 $aWEED CONTROL
700 1 $aBALZARINI, M.
700 1 $aZOPPOLO, R.
700 1 $aZARZA, H.
700 1 $aRODRÍGUEZ, E.M.
700 1 $aGRANVAL, N.I.
700 1 $aAMADO, I.
773 $tSustainable Agriculture Research, 2017$gv.6 (3): 26-34.
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